Table 2.
Cross-linking experiment plan for FGF-2
| Experiment | Cross-linker (length, Å) | Mutated residue | Single-experiment coverage | Cumulative coverage |
| 1 | DMP (9.2) | Arg 69 | 58.33% | 58.33% |
| 2 | BS3 (11.4) | Arg 116 | 53.85% | 69.23% |
| 3 | BS3 (11.4) | Arg 53 | 58.33% | 72.44% |
| 4 | Sulfo-EGS (16.1) | Arg 90 | 41.03% | 75.00% |
| 5 | DMP (9.2) | Arg 106 | 50.64% | 76.28% |
| 6 | Sulfo-EGS (16.1) | Arg 118 | 44.23% | 77.56% |
| 7 | DMP (9.2) | Asn 110 | 52.56% | 78.85% |
| 8 | DMP (9.2) | Arg 129 | 55.77% | 79.49% |
| Total | 79.49% |
The greedy set of eight experiments, each involving one possible Arg, Asn, Gln, or His to Lys mutation, and a choice of commercially available cross-linker, was determined. Each experiment is shown on a line, along with the coverage (percentage of directed model-pairs discriminated) at Δ = 12. The total coverage provided by all eight experiments is 79.49% of the 156 directed model pairs, which is very close to the plateau value of 80.13%.