Figure 1.

Sequence alignment of WT bovine chymotrypsin and rat-Δ A-chymotrypsin (lacking the propeptide). Boldface letters outline the differences in amino acid sequence. The three polypeptide chains (1–13, 16–146, 149–245) are indicated in different bars. The five inter- and intrachain disulfide bridges are shown by the black connections. Arrows below and above the sequence bar indicate the β-barrel structures and the structural elements such as segment2, loop6, and loop7, respectively.