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RNA-blot analysis of Hcf136 transcript accumulation. Approximately 5 μg of total RNA was fractionated on 1.5% agarose gels and transferred to nitrocellulose membrane. Separate filters were hybridized to radiolabeled fragments of Hcf136 (A) and Pepc and Rbcs (B). Ethidium bromide-stained 18S rRNA (Etbr) is shown as a loading control.
