Table IV.
Mol % of amino acids in seeds of Col wild type and mutants
The asterisk indicates a significant difference of amino acid content between the mutant and Col wild type (Dunnett's test, *, p < 0.05; **, p < 0.01; ***, p < 0.001).
| Amino Acida | Col Wild Type | 5-fcl | arc12 | ats1-1 | npq1-2 | sex1-1 | sex4-5 | tha1-1 | tt7-3 |
|---|---|---|---|---|---|---|---|---|---|
| Ala | 4.7 ± 0.1 | 1.6 ± 0.3*** | 5.1 ± 0.2 | 4.8 ± 0.3 | 4.8 ± 0.3 | 5.3 ± 0.2 | 5.4 ± 0.1 | 3.2 ± 0.2*** | 8.4 ± 0.8*** |
| Arg | 1.5 ± 0.1 | 2.7 ± 0.2** | 1.5 ± 0.1 | 1.6 ± 0.1 | 1.9 ± 0.2 | 1.7 ± 0.1 | 1.4 ± 0.1 | 1.5 ± 0.2 | 2.6 ± 0.3*** |
| Asn | 5.7 ± 0.1 | 1.3 ± 0.2*** | 6.3 ± 0.5 | 6.3 ± 0.7 | 5.5 ± 0.5 | 4.9 ± 0.2 | 4.7 ± 0.4 | 3.5 ± 0.3*** | 6.7 ± 1.0 |
| Asp | 9.0 ± 0.2 | 0.52 ± 0.03*** | 8.3 ± 0.3 | 8.0 ± 0.4 | 8.0 ± 0.9 | 7.9 ± 0.7 | 7.7 ± 0.8 | 3.4 ± 0.3*** | 6.3 ± 0.6*** |
| Cys | 0.043 ± 0.001 | 0.030 ± 0.004 | 0.040 ± 0.004 | 0.046 ± 0.005 | 0.041 ± 0.001 | 0.044 ± 0.004 | 0.048 ± 0.004 | 0.22 ± 0.01*** | 0.032 ± 0.002* |
| Gln | 2.9 ± 0.1 | 2.1 ± 0.3 | 2.7 ± 0.3 | 3.6 ± 0.8 | 3.4 ± 0.5 | 2.7 ± 0.2 | 2.9 ± 0.2 | 2.9 ± 0.5 | 3.0 ± 0.3 |
| Glu | 33 ± 1 | 4.5 ± 0.9*** | 34 ± 3 | 34 ± 4 | 29 ± 4 | 28 ± 2 | 32 ± 3 | 14 ± 1*** | 23 ± 2*** |
| Gly | 4.4 ± 0.1 | 41 ± 2*** | 4.3 ± 0.2 | 3.9 ± 0.1 | 4.2 ± 0.2 | 4.3 ± 0.1 | 3.9 ± 0.2 | 2.5 ± 0.2*** | 4.9 ± 0.4 |
| His | 1.6 ± 0.04 | 0.75 ± 0.07*** | 1.8 ± 0.2 | 1.8 ± 0.4 | 1.6 ± 0.04 | 2.0 ± 0.2 | 1.5 ± 0.1 | 1.5 ± 0.1 | 1.6 ± 0.1 |
| Ile | 2.0 ± 0.03 | 0.57 ± 0.05*** | 2.0 ± 0.1 | 2.2 ± 0.2 | 2.2 ± 0.3 | 2.1 ± 0.1 | 2.1 ± 0.1 | 1.3 ± 0.2*** | 3.0 ± 0.1*** |
| Leu | 2.6 ± 0.1 | 0.65 ± 0.05*** | 2.7 ± 0.2 | 2.7 ± 0.3 | 3.0 ± 0.5 | 2.9 ± 0.2 | 2.8 ± 0.1 | 1.7 ± 0.3* | 3.4 ± 0.1** |
| Lys | 1.4 ± 0.03 | 1.1 ± 0.2 | 1.5 ± 0.1 | 1.4 ± 0.2 | 1.7 ± 0.2* | 1.7 ± 0.1* | 1.5 ± 0.1 | 1.0 ± 0.05 | 1.6 ± 0.04 |
| Met | 0.52 ± 0.01 | 0.14 ± 0.01*** | 0.64 ± 0.03* | 0.58 ± 0.07 | 0.51 ± 0.06 | 0.52 ± 0.03 | 0.60 ± 0.05 | 0.28 ± 0.03*** | 0.57 ± 0.05 |
| Phe | 2.6 ± 0.03 | 0.74 ± 0.03*** | 2.7 ± 0.1 | 2.7 ± 0.1 | 2.9 ± 0.2 | 2.7 ± 0.1 | 2.7 ± 0.1 | 1.6 ± 0.1*** | 2.5 ± 0.1 |
| Pro | 12 ± 1 | 1.9 ± 0.1* | 9.8 ± 3.1 | 10 ± 4 | 14 ± 3 | 16 ± 3 | 13 ± 4 | 7.4 ± 2.6 | 13 ± 2 |
| Ser | 4.4 ± 0.1 | 36 ± 2*** | 5.0 ± 0.2 | 4.3 ± 0.1 | 4.5 ± 0.3 | 5.0 ± 0.4 | 5.1 ± 0.2 | 2.5 ± 0.1*** | 5.4 ± 0.2** |
| Thr | 3.8 ± 0.1 | 2.5 ± 0.1 | 4.2 ± 0.3 | 3.7 ± 0.4 | 4.0 ± 0.2 | 4.1 ± 0.2 | 3.9 ± 0.2 | 48 ± 3*** | 4.0 ± 0.3 |
| Trp | 2.6 ± 0.1 | 0.34 ± 0.01*** | 2.8 ± 0.1 | 2.4 ± 0.1 | 2.6 ± 0.2 | 2.8 ± 0.2 | 3.6 ± 0.3*** | 1.2 ± 0.1*** | 3.1 ± 0.3 |
| Tyr | 0.73 ± 0.01 | 0.20 ± 0.01*** | 0.82 ± 0.04 | 0.79 ± 0.02 | 0.81 ± 0.03 | 0.78 ± 0.01 | 0.77 ± 0.05 | 0.45 ± 0.03*** | 0.80 ± 0.04 |
| Val | 3.3 ± 0.1 | 1.1 ± 0.1*** | 3.1 ± 0.1 | 3.5 ± 0.1 | 3.8 ± 0.5 | 4.0 ± 0.2* | 3.2 ± 0.2 | 1.9 ± 0.4*** | 5.1 ± 0.2*** |
a
Data are presented as mean ± se (n = 3–6 for mutants, n = 67 for Col wild type). GABA, Ho-Ser, and Hyp were included in calculating the mol % of above amino acids.