Table V.

Mol % of amino acids in seeds of Ws wild type and mutants

The asterisk indicates a significant difference of amino acid content between the mutant and Ws wild type (Dunnett's test, *, p < 0.05; **, p < 0.01; ***, p < 0.001).

Amino Acida	Ws Wild Type	arc10	dpe2-1	fatb-ko	lkr-sdh	pig1-1
Ala	5.7 ± 0.1	5.5 ± 0.2	7.0 ± 0.5*	5.3 ± 0.6	6.9 ± 0.5	3.7 ± 0.5***
Arg	1.6 ± 0.1	1.7 ± 0.1	1.7 ± 0.2	2.2 ± 0.1**	1.8 ± 0.2	1.2 ± 0.1
Asn	4.4 ± 0.1	4.3 ± 0.2	3.6 ± 0.3	5.9 ± 0.4***	5.0 ± 0.3	5.4 ± 0.3**
Asp	9.1 ± 0.2	8.2 ± 0.4	8.0 ± 0.7	6.5 ± 0.3***	7.5 ± 0.4	6.8 ± 0.6**
Cys	0.052 ± 0.003	0.058 ± 0.005	0.045 ± 0.004	0.050 ± 0.003	0.049 ± 0.003	0.046 ± 0.004
Gln	2.9 ± 0.2	2.9 ± 0.5	5.0 ± 1.3	2.5 ± 0.1	2.7 ± 0.3	10 ± 2***
Glu	36 ± 1	35 ± 2	32 ± 1	31 ± 2	28 ± 1*	23 ± 1***
Gly	4.1 ± 0.1	4.0 ± 0.3	3.8 ± 0.5	4.2 ± 0.2	4.7 ± 0.4	4.4 ± 0.4
His	1.2 ± 0.02	1.2 ± 0.1	1.3 ± 0.1	1.8 ± 0.1***	1.4 ± 0.03	3.0 ± 0.3***
Ile	2.4 ± 0.04	2.6 ± 0.1	2.3 ± 0.1	2.3 ± 0.1	2.7 ± 0.1	6.0 ± 0.4***
Leu	2.8 ± 0.1	3.0 ± 0.2	3.4 ± 0.2	2.9 ± 0.2	3.5 ± 0.2*	4.8 ± 0.2***
Lys	1.4 ± 0.04	1.4 ± 0.1	1.7 ± 0.1	1.7 ± 0.1	3.5 ± 0.04***	1.4 ± 0.1
Met	0.60 ± 0.02	0.65 ± 0.03	0.58 ± 0.05	0.68 ± 0.04	0.59 ± 0.07	0.39 ± 0.06***
Phe	2.8 ± 0.03	2.9 ± 0.1	3.1 ± 0.2	3.2 ± 0.1**	3.0 ± 0.1	2.4 ± 0.2**
Pro	5.7 ± 0.9	4.8 ± 0.9	4.1 ± 0.3	11 ± 3	7.4 ± 1.4	8.0 ± 0.8
Ser	5.0 ± 0.1	4.9 ± 0.2	4.8 ± 0.5	5.7 ± 0.4*	5.6 ± 0.1	3.6 ± 0.2***
Thr	3.3 ± 0.1	3.1 ± 0.1	3.8 ± 0.3	3.4 ± 0.2	3.8 ± 0.2	5.3 ± 0.4***
Trp	6.2 ± 0.2	8.8 ± 0.3***	7.9 ± 0.7	5.4 ± 0.4	6.4 ± 0.7	1.4 ± 0.1***
Tyr	0.95 ± 0.01	0.99 ± 0.03	1.1 ± 0.05	0.79 ± 0.03***	0.89 ± 0.01	1.0 ± 0.1
Val	3.5 ± 0.05	3.4 ± 0.1	3.6 ± 0.2	3.1 ± 0.03	3.5 ± 0.2	6.7 ± 0.8***

^aData are presented as mean ± se (n = 3–6 for mutants, n = 33 for Ws wild type). GABA, Ho-Ser, and Hyp were included in calculating the mol % of above amino acids.