Figure 5.

Organellar targeting of plant types 1 and 2 COG2154 proteins fused to GFP. Chlorophyll autofluorescence (left) and GFP fluorescence (middle) are merged in the right-hand segments. A, Transient expression in Arabidopsis protoplasts of GFP fused to the type 1 protein of Arabidopsis (At1g29810, At1, fourth row) or Pinus (Pt1, fifth row). The top three rows show controls: cytosolic expression of GFP alone (top row), chloroplastic expression of GFP fused to the transit peptide of the Rubisco small subunit (second row), and mitochondrial expression of GFP fused to hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase (third row). Fluorescence was observed with an epifluorescence microscope. B, Confocal laser scanning micrographs of mesophyll protoplasts isolated from leaves of transgenic Arabidopsis plants expressing GFP fused to the type 2 protein of Arabidopsis (At5g51110, At2).