Table III.

Metabolite content determined by GC-MS of plants lacking NAD-ME activity

Values are mean ± se of four different biological replicas, each consisting of four determinations made on eight plants. The values are relative to the respective wild type (each metabolite = 1; the corresponding complete data set of metabolites is available in Supplemental Table S1). Those values that are significantly different from the respective wild type as determined by the Student's t test (P < 0.05) are in bold type.

	nad1 × 2
	End of the Day	End of the Night
Ala	0.98 ± 0.21	1.34 ± 0.13
Asn	0.88 ± 0.16	2.52 ± 0.35
Asp	0.87 ± 0.09	1.29 ± 0.10
γ-Aminobutyrate	1.00 ± 0.09	1.91 ± 0.22
Glu	0.92 ± 0.07	1.40 ± 0.12
Gln	0.89 ± 0.15	3.51 ± 0.40
Gly	0.87 ± 0.11	1.78 ± 0.23
His	1.13 ± 0.26	1.63 ± 0.18
Ile	0.87 ± 0.11	1.15 ± 0.15
Leu	0.81 ± 0.11	1.15 ± 0.16
Lys	1.26 ± 0.18	1.89 ± 0.29
Met	1.26 ± 0.08	1.32 ± 0.10
Phe	0.86 ± 0.08	1.25 ± 0.11
Pro	0.81 ± 0.12	1.63 ± 0.40
Ser	0.92 ± 0.09	1.96 ± 0.20
Thr	1.13 ± 0.07	1.47 ± 0.14
Val	1.07 ± 0.09	1.23 ± 0.15
Citrate	0.60 ± 0.13	0.72 ± 0.07
2-Oxoglutarate	0.75 ± 0.06	1.30 ± 0.05
Succinate	0.82 ± 0.06	1.43 ± 0.15
Fumarate	0.84 ± 0.11	0.57 ± 0.08
Malate	0.84 ± 0.12	1.13 ± 0.07
OAA	1.18 ± 0.13	0.87 ± 0.14
Pyruvate	0.76 ± 0.11	0.91 ± 0.13
Ara	1.08 ± 0.07	0.92 ± 0.08
Ascorbate	1.43 ± 0.25	1.03 ± 0.30
Fru	1.43 ± 0.18	1.06 ± 0.22
Gal	1.77 ± 0.19	0.71 ± 0.10
Glc	1.57 ± 0.12	1.12 ± 0.17
Man	1.33 ± 0.17	0.73 ± 0.11
Rib	0.83 ± 0.16	1.64 ± 0.24
Suc	1.42 ± 0.13	0.98 ± 0.06
3-PGA	1.14 ± 0.20	1.11 ± 0.10
DHAP	0.76 ± 0.12	1.41 ± 0.20
Lactate	1.01 ± 0.17	1.47 ± 0.27
Glycerate	0.98 ± 0.09	1.14 ± 0.15
Glycerol	1.26 ± 0.08	1.39 ± 0.22
Glycolate	1.56 ± 0.39	1.09 ± 0.43
Glyoxylate	1.22 ± 0.14	1.07 ± 0.11