Figure 3.

Visualization of intracellular Ca²⁺ following Lat-B treatment. A pollen tube loaded with fura-2-dextran shows a clear Ca²⁺ gradient (A). However, addition of Lat (2 nm) decreased the Ca²⁺ gradient slightly after 5 min (B), which became much more evident by 10 min (C). After 20 min the Ca²⁺ gradient has almost disappeared, and growth has stopped (D). Removal of Lat-B allowed recovery of growth and the tip-focused Ca²⁺ gradient, which became evident within 15 min (E). Scale bar is 10 μm.