Figure 5.

Mechanical impedance alters auxin response in Arabidopsis roots. A and B, Expression of DR5-GUS (A) and IAA2-GUS (B) in horizontally and vertically grown roots. C and D, Real-time PCR analyses of the expression of IAA17 (C) and IAA14 (D) genes in horizontally or vertically grown roots. Relative expression was calculated as described in Figure 4. Four-day-old DR5-GUS or IAA2-GUS seedlings were stained in a buffer containing 1 mm X-gluc for 15 and 1 h, respectively, at 37°C in the dark. These are representative images of 30 seedlings stained in two separate runs. Bar = 100 μm.