Figure 2.

Gain-of-function analysis for Cu responsiveness in transgenic moss plants. A, Schematic diagram of constructs. The fragments of the FeSOD promoter were inserted into upstream of rice actin promoter (pACTIN). F12 contains 120-bp DNA (−318 to −199). F1 and F2 contain 60-bp DNAs from −318 to −259 and from −258 to −199, respectively. 2 × F1 and 2 × F2 contain two tandem copies of 60-bp fragments (−318 to −259 or −258 to −199), respectively. B, GUS activities of the transgenic moss plants treated with or without CuSO₄. The bars represent the GUS activities of transgenic moss plants cultured in the presence of 2.0 μm CuSO₄. The GUS activities of the transgenic plants cultured in the absence of CuSO₄ were arbitrarily set to 1.0 (dotted line). The actin promoter-GUS (ACTIN) was used as a control. For each construct, at least three independent transgenic plants were examined. The bars indicate ± sd.