Figure 6.

Genetic complementation of sgl1 mutants by LFY. A, Homozygous sgl1-1 mutant plants transformed with SGL1:LFY, all adult leaves exhibiting wild-type compound leaf morphology. B, Wild-type-like flowers were developed from secondary inflorescence from axils of compound leaves of sgl1 SGL1:LFY transgenic lines. C, PCR-based genotyping of sgl1 SGL1:LFY transgenic lines indicated that three independent transgenic lines were homozygous for Tnt1 insertion in SGL1 gene (lanes 1–3) in contrast to the wild-type M. truncatula (ecotype R108), where no Tnt1 insertion was detected in SGL1 gene (lane 4). Top segment for detecting Tnt1 inserts; bottom for detecting Tnt1 insertion in SGL1. D, RT-PCR analysis of expression of LFY gene in sgl1 SGL1:LFY transgenic lines. Shown were LFY expression in two independent sgl1 SGL1:LFY transgenic lines (lanes 1 and 2), sgl1-1 mutant (lane 3), and wild-type M. truncatula (lane 4). Expression of an Actin gene was used as an internal loading control (bottom). E, A typical mature flower of wild-type M. truncatula (ecotype R108), exhibiting bilateral symmetric zygomorphic morphology. F to I, Floral organs of wild-type M. truncatula were dissected. Shown were a top view of vexillum (F), top (G), and side (H) views of keel and alae, the central carpel enclosed by the staminal tube, and a top view of dissected sepals (I). J, A representative mature flower of sgl1 SGL1:LFY transgenic lines, exhibiting wild-type-like morphology. K to N, Floral organs of sgl1 SGL1:LFY transgenic lines were dissected. Shown were a top view of vexillum (K), top (L) and side (M) views of keel and alae, the central carpel, and a top view of dissected sepals (N).