Figure 6.
Expression of defense-related genes in the ethylene-insensitive Nr mutant relative to that in wild-type Ailsa Craig plants. Four-week-old Nr and wild-type plants were inoculated with a suspension of Cmm bacteria (108 cfu/mL) or mock inoculated. Total RNA was extracted from leaf samples collected at 8 dpi and used to perform qRT-PCR analysis using primers specific for the following 17 Cmm-modulated defense genes (Supplemental Table S2): 1, PR P6; 2, chitinase class 2; 3, acidic 26-kD endochitinase; 4, protease inhibitor; 5, Pti5; 6, wound-induced protease inhibitor II; 7, Verticillium wilt R protein; 8, cysteine protease; 9, extensin; 10, dioxygenase E8; 11, PR P4; 12, PR P2; 13, chitinase; 14, EIX receptor 1; 15, wound-induced protein Sn-1; 16, osmotin-like protein; 17, patatin-like protein 3. Gene expression levels in Nr plants were calculated relative to those in wild-type plants for mock-inoculated (A) and Cmm-infected plants (B). Values are average ± se (n = 4). Genes, whose expression was significantly different (P < 0.05) in Nr than in wild-type plants according to a two-sided one sample t test, are marked with an asterisk.