Table I.
Validation of microarray data by quantitative RT-PCR for 11 up-regulated and four down-regulated genes
| GenBank Accession | Gene Product | Array Ratioa (Cmm/Mock) | qRT-PCR Ratiob (Cmm/Mock) |
|---|---|---|---|
| U89256 | Pti5, ERF/AP2 transcription factor | 163 | 193 |
| AF272366 | Verticillium wilt disease R protein | 88 | 3 |
| AI776170 | Cys protease | 81 | 116 |
| AJ133600 | Extensin | 20 | 105 |
| BG629612 | Chitinase | 55 | 317 |
| AY359965 | EIX receptor 1 | 10 | 9 |
| AW219676 | Wound-induced protein Sn-1 | 8 | 21 |
| AY093595 | Osmotin-like protein | 10 | 2 |
| AW032581 | KNOTTED1 transcription factor | 9 | 24 |
| BG629373 | Zeatin glucosyl transferase | 6 | 6 |
| CN385925 | Patatin-like protein 3 | 5 | 4 |
| AW622607 | Nitrate reductase | 0.5 | 0.6 |
| AW647641 | Peroxidase | 0.5 | 0.9 |
| BG630282 | Probable protein kinase | 0.4 | 0.4 |
| BF176599 | Pro-rich protein | 0.2 | 0.2 |
a
Ratio between expression in Cmm- and mock-inoculated stems as estimated by microarray analysis at 8 dpi. Data represent the mean of two biological replicates
b
Ratio between expression in Cmm- and mock-inoculated stems as estimated by qRT-PCR at 8 dpi. Data represent the mean of three biological replicates.