Table II.
Sequences of primers for real-time PCR
Gene-specific primers were designed with the PCR primer design tool primer premier 5.0 according to cDNA sequences obtained from NCBI GenBank (http://www.ncbi.nlm.nih.gov). Genes for real-time PCR were screened based on the results of semiquantitative RT-PCR.
| Gene | Specific Primers Used for Real-Time PCR
|
Expected Size | Accession No. | |
|---|---|---|---|---|
| Forward Primer (5′-3′) | Reverse Primer (5′-3′) | |||
| bp | ||||
| OsGSL1 | TGAGGACCTGCCACGATT | CACGCTGATTGCGAACAT | 119 | AP001389 |
| OsGSL3 | TGGCAAGCGACCACATAG | AGACCTTAGCACGGACTG | 283 | AP003268 |
| OsGSL5 | GTGGTGTCCCTGCTATGA | GTTGTTTGCTATTCTCCC | 185 | AP008212 |
| Osg1 | GGCGTATGGGACAAAGGA | TTCAGAGGCGAAGGATGG | 237 | AB070742 |
| Gns5 | TTGCGGCCATTCCTACAGT | TGGTGAGGGCGATGCTTG | 183 | U72251 |
| Actin1 | CAGCACATTCCAGCAGAT | GGCTTAGCATTCTTGGGT | 108 | AB047313 |