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. 2008 Apr;146(4):1909–1919. doi: 10.1104/pp.107.111500

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Copyright © 2008, American Society of Plant Biologists

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Figure 10. — Expression and purification of the recombinant protein. In the expression of Trx-Gb-nsLTP1 fusion protein, lanes 1 and 2 indicate a soluble fraction of the cell lysate before and after induction with 0.5 mm isopropylthio-β-galactoside, respectively. Lane 3 shows a fraction with Trx-Gb-nsLTP1 fusion protein after Ni-NTA column chromatography; lane 4 is indicative of a fraction with HRV3C protease treatment. Lane 5 indicates the recombinant protein purified by Mono-S chromatography. Molecular mass markers are shown in lane M. The positions of Trx-Gb-nsLTP1 and Gb-nsLTP1 are marked by thin and thick arrows, respectively.