Influence of cis-unsaturated fatty acids on the fluorescence level of the mutated Gb-nsLTP1-TNS complex. The percentage of fluorescence of the complex toward the wild-type, R46A, P79A, F80A, and I82A proteins indicates black, white, light gray, gray, and dark gray bars, respectively. Experiments were performed in triplicate and results are expressed as the mean values ± sds.