Table II.
Differentially accumulated transcripts annotated as related to N assimilation
| Target Identificationa | Annotation | Rootsb
|
Shootsb
|
|||||
|---|---|---|---|---|---|---|---|---|
| LNO3− versus NO3− | LNO3− versus SNO3− | LNH4+ versus SNH4+ | LN2 versus SN2 | LNO3− versus SNO3− | LNH4+ versus SNH4+ | LN2 versus SN2 | ||
| Nitrate and Nitrite Reduction | ||||||||
| Msa.1381.1.S1_at | Nitrate reductase NADH | 6.06 | 9.19 | – | – | −2.13 | −4.38 | – |
| Mtr.10604.1.S1_at | Nitrate reductase [NADH] | 10.27 | 7.59 | – | 2.05 | – | −4.16 | – |
| Mtr.42446.1.S1_at | Nitrate reductase | 130.69 | 65.57 | – | 4.23 | −3.59 | −3.52 | 4.94 |
| Mtr.8568.1.S1_at | Nitrite reductase | 13.09 | 2.91 | – | – | −2.66 | −13.13 | |
| Mtr.13053.1.S1_at | Urophorphyrin III methylase | 29.86 | 5.21 | – | – | 5.58 | −15.83 | −10.78 |
| Mtr.13053.1.S1_s_at | Urophorphyrin III methylase | 12.17 | 3.72 | – | – | 4.66 | −11.31 | −9.55 |
| Mtr.22364.1.S1_at | Urophorphyrin III methylase | 3.25 | 3.16 | – | – | – | – | – |
| Mtr.44855.1.S1_at | Urophorphyrin III methylase | 14.88 | 4.56 | – | – | 4.13 | −9.88 | −12.51 |
| Mtr.37556.1.S1_at | Ferredoxin-NADP reductase | 10.82 | 2.65 | – | – | 2.30 | −2.36 | −3.48 |
| Mtr.40420.1.S1_at | Nonphotosynthetic ferredoxin | 23.92 | 3.01 | – | 2.83 | −8.31 | −22.55 | −60.97 |
| Mtr.39504.1.S1_at | Glc-6-P 1-dehydrogenase | 11.43 | 4.06 | – | – | 2.11 | −2.60 | −2.16 |
| Msa.2779.1.S1_at | 6-Phosphogluconate dehydrogenase | 4.81 | 2.66 | – | – | – | – | −2.34 |
| Mtr.43234.1.S1_at | 6-Phosphogluconate dehydrogenase | 4.30 | 2.37 | – | – | – | – | −2.09 |
| Msa.2673.1.S1_at | Transaldolase | – | – | – | – | – | – | −2.66 |
| Ammonium Assimilation | ||||||||
| Mtr.4818.1.S1_s_at | Gln synthetase | 9.88 | 3.07 | – | – | – | – | – |
| Msa.1654.1.S1_at | Gln synthetase | 9.38 | 2.97 | – | – | – | – | – |
| Mtr.10480.1.S1_at | Gln synthetase | 12.55 | 3.16 | – | – | – | – | – |
| Mtr.43850.1.S1_at | Mt N6/Gln synthetase I-like | – | – | – | 3.72 | – | – | – |
| Mtr.12432.1.S1_at | NADH-dependent Glu synthase | 2.06 | 2.17 | – | – | – | – | – |
| Mtr.42795.1.S1_at | Glu synthase | −2.98 | – | – | – | – | −2.03 | – |
| Mtr.7084.1.S1_at | Asn synthase | 5.50 | 3.45 | – | – | −4.87 | −3.75 | −11.20 |
| Mtr.7558.1.S1_at | Asn synthetase 2 | 3.51 | 2.85 | – | – | −3.02 | −3.22 | −7.57 |
| Mtr.33541.1.S1_x_at | Asn synthase | 6.52 | 3.62 | – | – | – | – | 7.65 |
| Glycolysis and Organic Acid Metabolism | ||||||||
| Mtr.40930.1.S1_at | Phosphoglycerate mutase 1 | 7.92 | 2.82 | – | – | – | – | – |
| Msa.1072.1.S1_at | Phosphoenolpyruvate carboxylase | – | – | – | – | – | – | 3.10 |
| Mtr.10198.1.S1_at | Phosphoenolpyruvate carboxylase | – | – | – | – | 2.71 | – | 3.88 |
| Mtr.13967.1.S1_at | Phosphoenolpyruvate carboxylase | – | – | – | – | – | – | 3.41 |
| Mtr.34902.1.S1_s_at | Phosphoenolpyruvate carboxylase | 66.49 | 7.89 | – | −2.24 | −3.29 | −10.70 | −6.80 |
| Mtr.36140.1.S1_at | Phosphoenolpyruvate carboxylase | – | – | – | – | – | – | 2.82 |
| Mtr.39390.1.S1_at | Phosphoenolpyruvate carboxylase | – | – | – | – | – | – | 3.15 |
| Mtr.8683.1.S1_at | Phosphoenolpyruvate carboxylase | 59.10 | 8.88 | – | – | −2.43 | −7.36 | −4.89 |
| Msa.3137.1.S1_at | Malate dehydrogenase | 2.09 | 2.17 | – | – | – | – | – |
| Mtr.40396.1.S1_at | Malate dehydrogenase | 2.02 | 2.20 | – | 2.07 | – | – | – |
| Mtr.45179.1.S1_at | Malate dehydrogenase | 2.58 | – | −2.64 | – | – | – | – |
| Mtr.6743.1.S1_at | 2-Oxoglutarate/malatetranslocator-like | – | – | – | 2.16 | – | −2.00 | – |
| Putative Nitrate Transporters | ||||||||
| Mtr.44730.1.S1_at | NRT1 transporter similar to AtNRT1.4 | – | – | – | −7.36 | – | – | – |
| Mtr.39005.1.S1_at | NRT1 transporter similar to AtNRT1.4 | – | – | – | −7.97 | – | – | – |
| Msa.3151.1.S1_at | NRT1 transporter similar to AtNRT1.4 | – | – | – | −6.06 | – | – | – |
| Mtr.35838.1.S1_at | NRT1 transporter similar to AtNRT1.1 | 35.51 | 3.22 | −2.69 | −5.13 | 1.82 | 1.56 | 2.28 |
| Mtr.5369.1.S1_at | NRT1 transporter similar to AtNRT1.1 | 69.07 | 4.71 | −2.52 | −9.82 | 1.87 | 1.34 | 2.45 |
| Mtr.27575.1.S1_at | NRT1 transporter similar to AtNRT1.1 | 61.61 | 4.55 | −2.58 | −7.06 | – | – | 2.59 |
| Mtr.40975.1.S1_at | NRT1 transporter | – | – | 2.28 | – | – | – | – |
| Mtr.37657.1.S1_at | NRT1 transporter | – | – | – | 2.26 | −3.80 | −3.38 | – |
| Mtr.35456.1.S1_at | NRT2 transporter similar to AtNRT2.5 | −12.77 | 2.25 | 3.75 | 14.52 | – | – | – |
| Mtr.40270.1.S1_at | NRT2 transporter similar to AtNRT2.1 | 2.07 | 4.94 | – | – | – | – | – |
| Mtr.9576.1.S1_at | CLC channel (similar clc-Nt2) | 2.73 | – | – | – | −2.63 | −10.20 | −102.54 |
| Mtr.39260.1.S1_at | CLC channel (similar to Atclc-b) | 2.55 | – | – | – | −2.87 | −10.78 | −43.26 |
| Mtr.32338.1.S1_at | CLC channel (similar to Atclc-e) | −2.50 | – | – | – | – | – | – |
| Putative Ammonium Transporters | ||||||||
| Mtr.10556.1.S1_at | Putative AMT1 transporter | – | – | – | – | – | – | – |
| Mtr.3650.1.S1_at | Putative AMT1 transporter | – | – | – | – | – | – | – |
| Mtr.1706.1S1_at | Putative AMT1 transporter | – | – | – | – | – | – | – |
| Mtr.46839.1.S1_at | Putative AMT1 transporter | – | – | – | – | – | – | – |
| Mtr.19853.1.S1_at | Putative AMT2 transporter | – | – | – | – | – | – | – |
| Mtr.32395.1.S1_s_at | Putative AMT2 transporter | – | – | – | – | – | – | – |
| Mtr.43740.1.S1_at | Nodulin 26-like protein (MIP family) | – | – | – | 19.03 | – | – | – |
| Msa.1751.1.S1_at | Nodulin 26-like protein (MIP family) | – | – | – | 4.17 | – | – | – |
a
Target identifier (Affymetrix or MtTF Q-RT-PCR).
b
Fold-change of transcript accumulation.