Figure 8.

Effect of glycerol on NRT2.1, NRT1.1, NRAMP4, and AKT1 expression and on G6P level in roots. At the end of a normal night, the plants were either transferred for 4 h in the light (L), for 4 h in the light with supply of 30 mm glycerol (L+Gly), or kept in the dark for 4 additional h (D). All transcripts were measured using real-time quantitative PCR and normalized to a putative clathrin coat-assembly protein gene (At4g24550). Concentrations of G6P were determined by cycling assays. The data are representative of at least two independent experiments (three replicates from each experiment).