Figure 6. Voltage dependence of TRPV3RK^- mutant in response to repetitive 2APB stimulations.

A, a representative experiment performed as in Fig. 3A except that the cell expressed TRPV3RK^-. B and C, G-V relationships for TRPV3RK^- were obtained from tail currents as in Figs. 3C and 3D. No significant change was found between the EGTA and BAPTA internal solutions except for some small changes in VDF. * p < 0.05 different from BAPTA; ^# p < 0.05 different from the first stimulation. D and E, peak current densities (D) and τ_act (E) at -100 and +100 mV elicited by the first (left panels) and 23^rd (right panels) applications of 100 μM 2APB to HEK293 cells that expressed TRPV3RK^- (RK^-). Data obtained from wild type TRPV3 (WT) for the 23^rd stimulation are included for comparison. The internal solution contained either 1 mM EGTA (open bars) or 10 mM BAPTA (filled bars). Currents were recorded using the voltage ramp protocol. τ_act was obtained by fitting the data points from 0.5 to 12 sec (or the time at the peak current) following 2APB application. n = 5-11 cells. * p < 0.01 different from WT/EGTA, ^† p < 0.05 different from WT/BAPTA.