Figure 5. Heat-evoked activity in oocytes that expressed TRPV3 and the G573S mutant.

A, representative current traces at -100 mV for uninjected control (Cntl) and oocytes injected with cRNA for wild type TRPV3 (WT), WT plus G573S (G/S), and G/S alone in response to 300 μM 2APB (open bar), a temperature drop to 10°C, as well as a temperature ramp from 10°C to 40°C as indicated. Note a different scale is used for G/S alone than for other traces. B, I-V relationships for oocytes shown in A at 22°C, 22°C with 2APB, 10°C, and 40°C. C, current vs temperature plots for individual cells grouped by the injected cRNA. Note the large cell-to-cell variation within each group and the different scales used for currents for different groups. D, the same data in C were normalized to current at 40 °C. E, log current vs temperature plot for a representative oocyte that coexpressed WT and G/S. The intersection of the two linear fit lines gives the temperature threshold. Q₁₀ values are determined by the antilog of 10*slope (10¹⁰*^sploe). F, summary of response/basal (means ± SEM) for temperature rise from 22 to 40°C. * p < 0.05 different from control, † p < 0.05 different from WT+G/S.