Table 1.
PCR amplification results in negative control (Toulon), and plague-infected ancient teeth (Marseille, Lambesc)
| Origin of tooth
|
Targeted sequence
|
||||
|---|---|---|---|---|---|
| Location | Individual | Tooth | β-Globulin | rpoB | pla |
| Marseille | S61 | 46 | + | NT | + |
| 34 | NT | NT | + | ||
| 16 | NT | NT | + | ||
| S122 | 16 | NT | NT | + | |
| 75 | + | NT | − | ||
| 36 | NT | NT | − | ||
| 46 | NT | NT | − | ||
| S117 | 46 | NT | NT | − | |
| 34 | NT | NT | |||
| Lambesc | S19 | 33 | + | +* | + |
| 35 | NT | +* | − | ||
| S87 | 36 | + | +* | + | |
| 85 | NT | +* | − | ||
| Toulon | 1 | 35 | + | − | − |
| 2 | 47 | + | − | − | |
| 8 | 23 | + | NT | − | |
| 4 | 48 | + | − | − | |
| 5 | 35 | + | − | − | |
| 3 | 24 | + | NT | − | |
| 7 | 36 | + | NT | − | |
Teeth are numbered according to the international nomenclature. +, Positive PCR with first amplification; +*, PCR amplicon only visible following second round PCR; −, no amplicon; NT, not tested.