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. Author manuscript; available in PMC: 2009 Feb 20.

Published in final edited form as: Virology. 2007 Nov 5;371(2):257–266. doi: 10.1016/j.virol.2007.10.009

γ-secretase inhibitors are unable to inhibit LMP2A auto-regulation. HEK293 cells were transiently transfected with LMP2p and LMP2A in the presence of 1μM, 10 μM or 50 μM DAPT or DMSO vehicle control for 18 hours and lysed. Lysates were used to measure luciferase activity (A) or protein expression by Western blot (B). Luciferase activity for untreated cells was set to 100% and the luciferase activity of DMSO and inhibitor treated cells was normalized to this value. The average relative luciferase activity from three independent experiments is shown +/− standard error. There is no statistically significant difference between DMSO and inhibitor treated cells. Similar results were obtained using γ-secretase inhibitor XII (Z-IL-CHO), data not shown.

γ-secretase inhibitors are unable to inhibit LMP2A auto-regulation. HEK293 cells were transiently transfected with LMP2p and LMP2A in the presence of 1μM, 10 μM or 50 μM DAPT or DMSO vehicle control for 18 hours and lysed. Lysates were used to measure luciferase activity (A) or protein expression by Western blot (B). Luciferase activity for untreated cells was set to 100% and the luciferase activity of DMSO and inhibitor treated cells was normalized to this value. The average relative luciferase activity from three independent experiments is shown +/− standard error. There is no statistically significant difference between DMSO and inhibitor treated cells. Similar results were obtained using γ-secretase inhibitor XII (Z-IL-CHO), data not shown.