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. Author manuscript; available in PMC: 2009 Feb 20.

Published in final edited form as: Virology. 2007 Nov 5;371(2):257–266. doi: 10.1016/j.virol.2007.10.009

Signaling from the amino-terminus of LMP2A is required for promoter activation. A) LMP2p was transiently transfected into HEK293 with wild-type LMP2A-HA or various LMP2A point mutants as activators and analyzed for luciferase activity. Firefly luciferase values were normalized to an internal renilla luciferase control and fold induction calculated compared to no activator. Average fold induction from at least five independent experiments is shown +/− standard error. * indicates p<0.05 by student t-test. B) Whole cell lysates from HEK293 cells transfected with LMP2A-HA or LMP2A point mutants were used for Western blot analysis. Duplicate blots were probed with antibodies specific for LMP2A (14B7) or phospho-tyrosine (RC20-HRP). A representative blot of three independent experiments is shown.

Signaling from the amino-terminus of LMP2A is required for promoter activation. A) LMP2p was transiently transfected into HEK293 with wild-type LMP2A-HA or various LMP2A point mutants as activators and analyzed for luciferase activity. Firefly luciferase values were normalized to an internal renilla luciferase control and fold induction calculated compared to no activator. Average fold induction from at least five independent experiments is shown +/− standard error. * indicates p<0.05 by student t-test. B) Whole cell lysates from HEK293 cells transfected with LMP2A-HA or LMP2A point mutants were used for Western blot analysis. Duplicate blots were probed with antibodies specific for LMP2A (14B7) or phospho-tyrosine (RC20-HRP). A representative blot of three independent experiments is shown.