Figure 2.

NV1066 selectively induces enhanced green fluorescent protein (eGFP) expression in cancer cells but not in nerves in an in vitro NI model. Dorsal root ganglia neurons were grown in Matrigel adjacent to a colony of cancer cells. NV1066 (5 x 10⁷ PFU) was injected into the Matrigel 5 days after implantation. Microscopic images were acquired 48 hours after infection using bright field (A), fluorescence (B), and overlay (C) modes (original magnifications, x40). High-magnification images illustrate that eGFP is expressed electively by the cancer cells. Arrowheads indicate nerve cells. The virus had no visible toxic effects on the nerves.