Table 1.
Characteristics of microsatellite loci in Thryothororus pleurostictus and cross-species allele size ranges and number of alleles in Troglodytes aedon and Thryothorus rufalbus
| Thryothorus pleurostictus (banded wren) | Thryothorus rufalbus (rufous-and-white wren) | Troglodytes aedon (house wren) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Locus* | Repeat Motif | Ta (°C) | MgCl2 (mM) | Primer Sequence † | Size range (bp) | Na | n | Size range (bp) | Na | n | Size range (bp) | Na | n |
| ThPl-01 | (GT)8 | 62 | 1.5 | F:CTTTGGGGCAGTGTTGTGGAATG | 300–302 | 1 | 11 | 298–299 | 2 | 14 | 282–299 | 5 | 15 |
| R:GGCTGGCTGGGAGGCACAG | |||||||||||||
| ThPl-14 | (CA)5 (GACATACAGA) (CA)7 | 60 | 2 | F:GTAAATTTCAGGAGTCCAGGTTGC | 237–245 | 3 | 11 | 245–262 | 5 | 15 | 243–227 | 11 | 15 |
| R:AAGCGCCCAAAATTAGCCAGAA | |||||||||||||
| ThPl-15 | (GT)2 (GA) (GT)4 (GAGTAGT) | 60 | 3 | F:TTGTCTTCTTCTCAGTTTGTCTCA | 286 | 1 | 11 | 277 | 1 | 15 | 277 | 1 | 15 |
| R:GCGTTTGTGTTACTGAAGATTTAG | |||||||||||||
| ThPl-16 | (TG)8 | 60 | 3 | F:CACTCTTTGAATTAGCTCTCCTCA | 129–131 | 1 | 11 | 137 | 1 | 15 | N.A | - | 14 |
| R:GCAAAAACAAGATATCCTCAGTCC | |||||||||||||
| ThPl-17 | (GT)8 | 60 | 2 | F:AGTGGCTGGGTGTTCTTTCAT | 157 | 1 | 11 | 143–160 | 3 | 15 | 160–162 | 2 | 15 |
| R:CACATCCTTTCCCTCCTGGTA | |||||||||||||
| ThPl-20 | (GT)3 GA (GT)7 | 60 | 3 | F:CTTGCCATAGAATGCAGTTGAAT | 267 | 1 | 11 | 256 | 1 | 15 | 256 | 1 | 15 |
| R:TAGTTCCAGTCCTCTTCTTTTACC | |||||||||||||
| ThPl-22 | (GT)1(CT)1(GT)2(AT)1(GT)4(CT)1(GT)3 | 60 | 3 | F:GAGAAGAGTGCATAGGACAATCA | 140 | 1 | 11 | 132–141 | 3 | 14 | N.A | - | 15 |
| R:TGGTGGCACGTTACAGGTTT | |||||||||||||
| ThPl-26 | (GT)8 | 60 | 3 | F:TCAAATGTGCCACTGACTGAGT | 188 | 1 | 11 | 182–188 | 3 | 15 | 182 | 1 | 15 |
| R:AGCCTACTTCAAACTGAGACAGA | |||||||||||||
| ThPl-27 | (AC)15 | 60 | 2 | F:TCTCTGCGTCTGCTTGGTG | 175–196 | 3 | 11 | 180 | 1 | 14 | N.A | - | 15 |
| R:CTTCCTGGGATAGATAATGTGAC | |||||||||||||
| ThPl-30 | (TG)6 TA (TG)3 | 60 | 3 | F:ATGCCAGCACTAAAGAATGACAA | 221 | 1 | 11 | 227–252 | 7 | 13 | N.A | - | 15 |
| R:CTACATAGCAGGCAGCAGAGGTT | |||||||||||||
| ThPl-37 | (CAA)7 | 60 | 3 | F:CCATCAGATTCCTAAGATAAACC | 199–200 | 2 | 11 | N.A | - | 15 | N.A | - | 15 |
| R:TCCCAGCCCACCCTGTA | |||||||||||||