Table 1.
Identification of Nematostella vectensis counterparts of bilaterian Hedgehog signal transduction genes
|
N. vectensis Pathway Component |
Function |
Nv JGI Protein model ID (or EST ID) |
Human Homolog UniProt ID |
Drosophila homolog |
e value in BLASTp with human homolog |
|---|---|---|---|---|---|
| NvHint1 | unknown | this study | n/a | n/a | n/a |
| NvHint2 | unknown | this study | n/a | n/a | n/a |
| NvHint3 | unknown | this study | n/a | n/a | n/a |
| NvHh1 | ligand | this study | Q15465 Sonic Hh O43323 Desert Hh Q14623 Indian Hh | hedgehog | 0 |
| NvHh2 | ligand | this study | Q15465 Sonic Hh | hedgehog | 0 |
| NvSkinny Hh [rasp, sightless] | palmitoylation of Hh ligand | 217054 | Q17N53 | skinny Hh, sightless, central missing | 2 e-40 |
| NvDispatched | ligand transporter - sending cell | 199988 | Q96F81 | Q9U477 | 1 e-88 |
| NvGlypican5 | Hh movement, signaling range | 218206 | P35052 | dally-like UP:Q9GPL5 dally UP:Q966V5 | 8 e-32 |
| NvGlypican6 | Hh movement and signaling range | 218207, 247677 | Q8N158 | dally-like UP:Q9GPL5 | 7 e-92 |
| NvHIP | Hh binding membrane protein | 197846 | Q96QV1 | unknown | 1 e-40 |
| NvCdon/Boc | Hh-binding type I receptor | 185528, 105427 | Q4KMG0 (Cdon) Q9BWV1 (Boc) | iHog | Cdon 7e-30 Boc 4e-31 |
| NvGAS1 | GDNF-receptor-like Hh binding protein represses Hh activity/range | 104344 | unknown | 6 e-13 | |
| NvExostosin-1 | glycosyl transferase for heparan biosynthesis; Hh movement & reception | 173450 | Q16394 | tout-velut | 0 |
| NvExostosin-2 | glycosyl transferase for heparan biosynthesis; Hh movement & reception | 168451 | Q93063 | tout-velut | 0 |
| NvExostosin-3 | glycosyl transferase for heparan biosynthesis; Hh movement & reception | 235881 | O43909 | tout-velut | 0 |
| NvMegalin/gp330 | ligand binding and endocytosis | 196768 | Q7Z5C0 | CG12139 | 0 |
| NvPatched (Ptch) | 11 TM receptor binds Hh, represses Smo | this study | Q13635 | patched | 0 |
| NvSmoothened-1 (Smo1) | 7 TM receptor regulated by Ptch; interfaces with costal & downstream pathway | 208236 | Q99835 | smoothened | 2 e-138 |
| NvSmoothened-2 (Smo2) | 7 TM receptor regulated by Ptch; interfaces with intracellular pathway | 201646 | Q99835 | smoothened | 1 e-91 |
| Nvßarrestin1 | internalization of Smoothened | 97737 | kurtz | 3 e-111 | |
| Nvßarrestin2 | internalization of Smoothened | 149831 | kurtz | 8 e-14 | |
| NvGPRK2/ßARK-1 | G-protein coupled receptor kinase that targets Smoothened | 246204 | Q86YK6 | Gprk2, CG17998 | 0 |
| NvFKBP8 | peptidylprolyl isomerase, represses Smoothened | 91671 | Q7K3D4 | CG5482 | 7 e-34 |
| Nvrab23 | small GTPase for endocytosis & trafficking of Hh-receptor complexes | 25310 | Q9VNG6 | CG2108 | 2 e-64 |
| NvTectonic | signal transduction downstream of Rab23 | (JGI_CAGN8498) | Q2MV58 | unknown | 3 e-47 |
| NvKif3A | potential Costal 2 (Cos2) ortholog, Gli/fused scaffold interface with ciliary microtubules | 160820 | Q59EN1 | costal? | 0 |
| NvKif4A | potential Costal 2 (Cos2) ortholog, Gli/fused scaffold interface with ciliary microtubules | 233887 | O95239 | costal? | 3 e-131 |
| NvKIF27A | Costal 2 (Cos2) ortholog, Gli/fused scaffold interface with ciliary microtubules | 196177 | Q86VH2 | costal | 0 |
| NvFantom/Rpgrip1L | ciliary basal body protein required for Hh signal transduction | 181095 | Q68CZ1 | unknown | 0 |
| NvIft88/polaris/Tg737 | ciliary Hh signaling regulator | 40068 | Q13099 | nompB | 0 |
| NvIft172/wimple | ciliary Hh signaling regulator | (JGI_CAGF7669) | Q96HW4 | osm-1 | 2 e-99 |
| NvIft52/Ngd5 | ciliary Hh signaling regulator | 239245 | Q9Y366 | osm-6 | 2 e-158 |
| NvFused1 | protein kinase phosphorylates Cos2 | 219073 | Q9NRP7 | fused | 1 e-68 |
| NvFused2 | protein kinase - Gli regulation | 173313 | XP_950751* | fused | 0 |
| NvSuppressor of Fused, SuFu | binds and represses Gli | 246114 | Q9UMX1 | Su(Fu) | 6 e-120 |
| Su(Fu) | |||||
| Nvlguana | regulates Gli downstream of Smo, interacts with Sufu | 247454 | Q86YF9 | unknown | 2 e-23 |
| NvPKA | Gli phosphorylation/processing | 120559 | P22694 | Pka-C1, CG4379 | 2 e-100 |
| NvGSK3ß | Gli phosphorylation/processing | 11896 | P49841 | shaggy | 0 |
| NvCK1 | Smo and Gli phosphorylation/processing | 159193 | P48729 | gilgamesh | 4 e-152 |
| NvßTRCP1 | Fbox Ub ligase for Gli-R processing | 228088 | Q9Y297 | slimb | 0 |
| NvßTRCP2 | Fbox Ub ligase for Gli-R processing | 648 | Q9Y297 | slimb | 2 e-165 |
| NvSIL | signal transduction, function unclear | 246782 | Q15468 | unknown | 2 e-65 |
| NvGli3 | Transcriptional activator (Gli-full-length) or repressor (Gli-R) when cleaved | this study | P10071 | Cubitus interruptus (Ci) | 0 |
| NvSap18 | Gli partner: Histone deacetylase subunit:Sin3-associated polypeptide 18 | 226633 | O00422 | Bin1 | 5 e-41 |
| NvSPOP | Gli ubiquitylation and proteolysis | 177584 | O43791 | roadkill | 1 e-153 |
| NvTalpid3 | Gli regulation | (JGI_CAGG5027) | Q1G7G8** | unknown | 1 e-9** |
*
NCBI protein accession number
**
chick protein