Table 1.
Results of MALDI-TOF mass spectra and database searching for protein identification.
| Identified proteins | Spot(a) number | Mr(kDa)/pI(b) | Access N°(c) | Peptidesmatched | Relative(d)volume (%) | Mass accuracy (ppm) | Sequence coverage % | Reported function | |
|---|---|---|---|---|---|---|---|---|---|
| N | T | ||||||||
| Complement component C3c (chainD) | 1 | 71317/6.82 | 78101272 | 36 | 0.085 ± 0.015 | 0.02 ± 0.007 | 45 | 64 | Immune response |
| IGHG1 protein | 2 | 45286/8.63 | 44890623 | 9 | 0.121 ± 0.001 | 0.021± 0.008 | 42 | 27 | Immune response |
| IGHG1 protein | 3 | 45286/8.63 | 44890623 | 9 | 0.142 ± 0.008 | 0.041 ± 0.006 | 43 | 29 | Immune response |
| IGHG1 protein | 4 | 45286/8.63 | 44890623 | 7 | 0.12 ± 0.004 | 0.019 ± 0.009 | 50 | 24 | Immune response |
| IGHG1 protein | 5 | 45286/8.63 | 44890623 | 7 | 0.101 ± 0.006 | 0.014 ± 0.005 | 50 | 24 | Immune response |
| IGHG1 protein | 6 | 45286/8.63 | 44890623 | 7 | 0.095 ± 0.007 | 0.017 ± 0.006 | 50 | 24 | Immune response |
| Carbonic anhydrase 1 | 7 | 28909/6.59 | 4502517 | 13 | 0.081 ± 0.01 | 0.018 ± 0.009 | 72 | 72 | metabolic enzyme |
| Enolase1 | 8 | 47481/7.01 | 4503571 | 30 | 0.012 ± 0.001 | 0.131 ± 0.006 | 41 | 62 | Glycolytic enzyme |
| phosphoglycerate kinase 1 | 9 | 44973/8.30 | 48145549 | 28 | 0.015 ± 0.007 | 0.108 ± 0.03 | 60 | 58 | Glycolytic enzyme |
| Deoxy hemoglobin (ChainD) | 10 | 15928/7.14 | 27574253 | 11 | 0.018 ± 0.004 | 0.123 ± 0.006 | 49 | 79 | O2/CO2 transport |
| Deoxy hemoglobin (ChainD) | 11 | 15928/7.14 | 27574253 | 11 | 0.022 ± 0.002 | 0.131 ± 0.002 | 49 | 80 | O2/CO2 transport |
| Manganese superoxide dismutase | 12 | 22290/6.86 | 2780819 | 9 | 0.019 ± 0.006 | 0.075 ± 0.001 | 50 | 57 | Anti-oxidant enzyme |
| Alpha B-Crystallin | 13 | 20146/6.76 | 13937813 | 11 | 0.022 ± 0.001 | 0.097 ± 0.014 | 57 | 56 | Chaperone |
| Raf Kinase inhibitor protein | 14 | 16068/8,81 | 4261934 | 9 | 0.019 ± 0.008 | 0.132 ± 0.015 | 45 | 54 | Signal trans- duction |
| Peptidyl- prolylisomerase A | 15 | 18228/7,68 | 13937981 | 13 | 0.02 ± 0.007 | 0.109 ± 0.001 | 67 | 59 | Immuno- philin |
| Peptidyl- prolylisomerase A | 16 | 18228/7,68 | 13937981 | 14 | 0.011 ± 0.004 | 0.102 ± 0.005 | 67 | 60 | Immuno- philin |
| Cofilin1 | 17 | 18719/8.22 | 15147369 | 8 | 0.018 ± 0.008 | 0.112 ± 0.012 | 35 | 45 | Actin turnover |
| HSP27 | 18 | 22427/7.83 | 662841 | 12 | 0.021 ± 0.001 | 0.117 ± 0.003 | 48 | 51 | Chaperone |
| HSP27 | 19 | 22427/7.83 | 662841 | 16 | 0.014 ± 0.008 | 0.097 ± 0.005 | 48 | 67 | Chaperone |
| hnRNPA2/B1 isoform A2 | 20 | 36041/8.67 | 4504447 | 21 | 0.015 ± 0.007 | 0.141 ± 0.021 | 35 | 53 | Several functions |
(a)Index in the reference gel (Figure 1).
(b)Expected Mr and pI which were calculated on the complete sequences.
(c)Accession number in NCBI.
(d)The individual spot volumes were expressed as a percentage of the total volume in all of the spots present in the gel. N: nontumor tissue; T: tumor tissue.