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. 2008 Apr 3;2008:695432. doi: 10.1155/2008/695432

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Copyright © 2008 Ana M. Montalvo-Álvarez et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Analysis of protective effect of DNA vaccination with L. amazonensis HSP20 gene. (a) Expression of Leishmania HSP20 in pcDNA3-LaHSP20 transfected COS7 cells. COS7 cells were transiently transfected by electroporation with either pcDNA3 DNA (lane 1) or pcDNA3-LaHSP20 DNA (lane 2). Protein extracts were analyzed by 12% SDS-PAGE and stained with Coomassie blue (left panel). The expression of Leishmania HSP20 was assayed by Western blotting using a mouse anti-HSP20 polyclonal antibody (right panel). (b) BALB/c mice (six per group) were immunized twice at 2-week interval with PBS, empty vector or pcDNA3-LaHSP20. After challenge with L. amazonensis, lesion development (footpad swelling) was monitored weekly. Each point represents the average and standard deviations for the group.