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. Author manuscript; available in PMC: 2008 Apr 7.
Published in final edited form as: Breast Cancer Res Treat. 2006 Jul 19;101(2):161–174. doi: 10.1007/s10549-006-9286-9

Figure 2. Expression of Lex epitopes in MCF-7 breast carcinoma cells, PMN, and HUVEC.

Figure 2

Lex expression was analyzed by flow cytometry using two mAbs against nonsialylated-Lex, FC-2.15 and MCS-1, and a mAb anti-sialylated-Lex, CSLEX1, followed by incubation with FITC-conjugated goat anti-mouse Ig. Data are taken from one representative experiment. Filled peaks represent staining with the primary anti-Lex or anti-sLex antibodies followed by FITC-secondary antibody. Staining by isotype-matched mouse immunoglobulins is indicated in white (each non-specific isotype control followed by FITC-conjugated goat anti-mouse Ig). Data are represented as histograms of fluorescence intensity for 10,000 light scatter-gated events. Median values of fluorescence intensity (MFI) are indicated in each figure.