Figure 5. In vitro FC-2.15-induced PMN aggregation exhibits dose- and time-dependence.
Suspensions of PMN were incubated in different conditions: a) with different FC-2.15 concentrations for 1 h at 37°C; b) with 20 μg/ml FC-2.15 during different incubation times at 37°C. c) PMN aggregation was evaluated in the presence of different concentrations of FC-2.15, control IgM, MCS-1 or control IgG3. The percentage of aggregated cells was calculated for each condition as described under Methods. Determinations were performed by duplicate (bars: mean ± SD).
