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. Author manuscript; available in PMC: 2008 Apr 7.
Published in final edited form as: Breast Cancer Res Treat. 2006 Jul 19;101(2):161–174. doi: 10.1007/s10549-006-9286-9

Figure 6. In vitro aggregation of PMN by FC-2.15 appears to be partially mediated by β2-integrins, which express Lex.

Figure 6

a) Isolated PMN were preincubated in the absence (control) or the presence of mAb TS1/18, a β2-integrin blocking antibody. Then, the agglutination assay was performed in the presence of FC-2.15 (20 μg/ml), KIM185 (20 μg/ml) or PBS (control). Each determination was performed in duplicates in two independent experiments (bars: mean ± SD). b) PMN membrane extracts were immunoprecipitated with either MEM-170 mAb, MEM 48 mAb or isotype-matched immunoglobulins (not shown), and immunoprecipitates were subjected to SDS-PAGE and immunoblotting with either MEM-170, MEM-48 or FC-2.15 mAbs as indicated.