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. Author manuscript; available in PMC: 2009 Feb 20.

Published in final edited form as: Vaccine. 2008 Jan 10;26(8):1098–1110. doi: 10.1016/j.vaccine.2007.12.024

Neutralizing antibody (NAb) responses against different panels of pseudotyped viruses. (a) NAb titers against Tier 1/autologous viruses including a T-cell line adapted (TCLA) HIV-1 virus (MN) and five pseudotyped viruses each expressing one of the five autologous primary Env antigens included in the DP6−001 formulation. The neutralization assays were done in TZM-bl cells and the NAb titers were measured using individual volunteer sera from Groups A and B (N = 21) at 2 weeks after the 2^nd protein boost. (b)-(c) NAb titers determined by PhenoSense™ assay in U87 cells with sera from Groups A and B at two weeks after the 2nd protein boost against pseudotyped viruses expressing either (b) three relatively sensitive to neutralization Env antigens of subtype B (MN, NL4−3 and SF162) or (c) eleven additional primary Env antigens of subtypes A, B, C, D and E. A serum dilution of 1:20 was used as the cut-off to score the positive NAb (shown as the broken line in each graph). (d) NAb responses against Tier 2 pseudotyped viruses expressing primary Env from subtypes A, B and C (4 viruses for each subtype). Individual subject sera from Groups A and B were collected at two weeks post the 2nd protein boost. The percent neutralization was measured at 1:10 serum dilution. The neutralization assays were done in TZM-bl cells.

Neutralizing antibody (NAb) responses against different panels of pseudotyped viruses. (a) NAb titers against Tier 1/autologous viruses including a T-cell line adapted (TCLA) HIV-1 virus (MN) and five pseudotyped viruses each expressing one of the five autologous primary Env antigens included in the DP6−001 formulation. The neutralization assays were done in TZM-bl cells and the NAb titers were measured using individual volunteer sera from Groups A and B (N = 21) at 2 weeks after the 2^nd protein boost. (b)-(c) NAb titers determined by PhenoSense™ assay in U87 cells with sera from Groups A and B at two weeks after the 2nd protein boost against pseudotyped viruses expressing either (b) three relatively sensitive to neutralization Env antigens of subtype B (MN, NL4−3 and SF162) or (c) eleven additional primary Env antigens of subtypes A, B, C, D and E. A serum dilution of 1:20 was used as the cut-off to score the positive NAb (shown as the broken line in each graph). (d) NAb responses against Tier 2 pseudotyped viruses expressing primary Env from subtypes A, B and C (4 viruses for each subtype). Individual subject sera from Groups A and B were collected at two weeks post the 2nd protein boost. The percent neutralization was measured at 1:10 serum dilution. The neutralization assays were done in TZM-bl cells.