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C3 deposition on S. pneumoniae was facilitated by rabbit antibodies to PspA in a dose-dependent manner. Strains WU2 (PspA clade 2) and JY1119 (PspA-negative) were incubated with different concentrations of heat-inactivated rabbit anti-Rx1 immune serum followed by incubation with 10% baby rabbit complement. The proportion of bacteria with C3 deposited was determined by flow cytometry.
