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C3 deposition increased with increasing the post-vaccination serum concentration. Different amounts of pre-bleed and post-vaccination sera from clinical trial subjects 43, 90 and 117 were incubated with strain WU2. Subject 43 had received a placebo vaccination and subjects 90 and 117 had been vaccinated with 125 μg/dose of Rx1 protein. The proportion of bacteria with C3 deposition was determined by flow cytometry.
