Figure 5.

Effect of amino acids supplementation of the medium previously conditioned by culture with villous explants on peripheral blood mononuclear cell proliferation

Peripheral blood mononuclear cells were cultured for 72 h in medium previously conditioned with 1000 units ml⁻¹ interferon-γ and supplemented with indicated amino acids to the concentrations normally present in RPMI 1640 or vehicle (phosphate-buffered saline (PBS)). [³H]Thymidine incorporation was then determined as described in Methods. Culture was performed by adding 100 μl of peripheral blood mononuclear cells suspended in tryptophan-free medium to 100 μl of supplemented conditioned medium, and therefore tryptophan concentration in peripheral blood mononuclear cell culture was half of that in each medium. Data represent the mean ±s.d. of three separate experiments with quadruplicate assay.