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Percentage of CD4–positive and CD8–positive cells
| CD4 (% of total) | CD8 (% of total) | |||
|---|---|---|---|---|
| Non–conditioned | Conditioned | Non–conditioned | Conditioned | |
| PHA (−) | 4.57 | 3.90 | 11.57 | 8.95 |
| PHA (+) | 49.33 | 28.90 | 19.32 | 17.90 |
Peripheral blood mononuclear cells were cultured for 72h either in non–conditioned medium or in medium previously conditioned by culture of villous explants. Percentage of CD4–positive and CD8–positive cells was then determined as described in Methods.
