Figure 1.

Nicotine-induced LHRH release in bullfrog sympathetic ganglia. (A) The voltage traces were recorded from a C (a) and a B (b) cell. Superfusion of nicotine is indicated by the heavy bar. (B) Depolarizations were recorded in a B neuron in response to increasing concentrations of nicotine. For each application, 3 ml of nicotine was used. (C) Dose responses of nicotine-induced depolarizations. The number of cells tested for each dose of nicotine is given under each point. The error bars are SE. (D) The arrow (a) and arrowheads (b and a) indicate pressure–ejection of [d-Glp¹,d-Phe²,d-Trp^3,6]LHRH (100 μM) at 4 bars for 2, 5, and 20 s, respectively. The dotted lines indicate the resting potentials.