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. Author manuscript; available in PMC: 2008 Apr 7.
Published in final edited form as: Free Radic Biol Med. 2007 Sep 18;43(12):1639–1648. doi: 10.1016/j.freeradbiomed.2007.08.031

Figure 1.

Figure 1

Nitric oxide (NO) concentrations in conditioned medium after 2 h incubation of in vitro Cu adequate (Cu+/Cu+) and Cu deficient (Cu−/Cu−) embryo (A) and yolk sac (B) in the presence or absence of a NOS inhibitor (L-NMMA; 10 mM), or NOS agonists (VEGF; 1 μg/ml, acetylcholine (Ach); 1 mM, ionomycin; 100 μM). Values are expressed as mean ± SEM (n=6–10/group). * p < 0.05 versus Cu+/Cu+ controls.