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. 1996 May;34(5):1050–1057. doi: 10.1128/jcm.34.5.1050-1057.1996

Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus.

Y Pongsuwanna 1, K Taniguchi 1, M Chiwakul 1, T Urasawa 1, F Wakasugi 1, C Jayavasu 1, S Urasawa 1
PMCID: PMC228953  PMID: 8727874

Abstract

A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty-three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively. Two samples exhibited two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10, respectively. For one specimen, containing a serotype G10 virus (strain P343), virus was isolated in MA-104 cells, and the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotype (or VP4 genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.

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Selected References

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  1. Bellinzoni R. B., Mattion N. M., Matson D. O., Blackhall J., La Torre J. L., Scodeller E. A., Urasawa S., Taniguchi K., Estes M. K. Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. J Clin Microbiol. 1990 Mar;28(3):633–636. doi: 10.1128/jcm.28.3.633-636.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellinzoni R. C., Mattion N. M., Burrone O., Gonzalez A., La Torre J. L., Scodeller E. A. Isolation of group A swine rotaviruses displaying atypical electropherotypes. J Clin Microbiol. 1987 May;25(5):952–954. doi: 10.1128/jcm.25.5.952-954.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ciarlet M., Hidalgo M., Gorziglia M., Liprandi F. Characterization of neutralization epitopes on the VP7 surface protein of serotype G11 porcine rotaviruses. J Gen Virol. 1994 Aug;75(Pt 8):1867–1873. doi: 10.1099/0022-1317-75-8-1867. [DOI] [PubMed] [Google Scholar]
  4. Ciarlet M., Liprandi F. Serological and genomic characterization of two porcine rotaviruses with serotype G1 specificity. J Clin Microbiol. 1994 Jan;32(1):269–272. doi: 10.1128/jcm.32.1.269-272.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ciarlet M., Ludert J. E., Liprandi F. Comparative amino acid sequence analysis of the major outer capsid protein (VP7) of porcine rotaviruses with G3 and G5 serotype specificities isolated in Venezuela and Argentina. Arch Virol. 1995;140(3):437–451. doi: 10.1007/BF01718422. [DOI] [PubMed] [Google Scholar]
  6. Das M., Dunn S. J., Woode G. N., Greenberg H. B., Rao C. D. Both surface proteins (VP4 and VP7) of an asymptomatic neonatal rotavirus strain (I321) have high levels of sequence identity with the homologous proteins of a serotype 10 bovine rotavirus. Virology. 1993 May;194(1):374–379. doi: 10.1006/viro.1993.1271. [DOI] [PubMed] [Google Scholar]
  7. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flores J., Myslinski J., Kalica A. R., Greenberg H. B., Wyatt R. G., Kapikian A. Z., Chanock R. M. In vitro transcription of two human rotaviruses. J Virol. 1982 Sep;43(3):1032–1037. doi: 10.1128/jvi.43.3.1032-1037.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gatti M. S., de Castro A. F., Ferraz M. M., Fialho A. M., Pereira H. G. Viruses with bisegmented double-stranded RNA in pig faeces. Res Vet Sci. 1989 Nov;47(3):397–398. [PubMed] [Google Scholar]
  10. Gorziglia M., Hoshino Y., Buckler-White A., Blumentals I., Glass R., Flores J., Kapikian A. Z., Chanock R. M. Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7039–7043. doi: 10.1073/pnas.83.18.7039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gorziglia M., Nishikawa K., Hoshino Y., Taniguchi K. Similarity of the outer capsid protein VP4 of the Gottfried strain of porcine rotavirus to that of asymptomatic human rotavirus strains. J Virol. 1990 Jan;64(1):414–418. doi: 10.1128/jvi.64.1.414-418.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gouvea V., Glass R. I., Woods P., Taniguchi K., Clark H. F., Forrester B., Fang Z. Y. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol. 1990 Feb;28(2):276–282. doi: 10.1128/jcm.28.2.276-282.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gouvea V., Santos N., Timenetsky M. do C. Identification of bovine and porcine rotavirus G types by PCR. J Clin Microbiol. 1994 May;32(5):1338–1340. doi: 10.1128/jcm.32.5.1338-1340.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gouvea V., Santos N., Timenetsky M. do C. VP4 typing of bovine and porcine group A rotaviruses by PCR. J Clin Microbiol. 1994 May;32(5):1333–1337. doi: 10.1128/jcm.32.5.1333-1337.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gouvea V., de Castro L., Timenetsky M. C., Greenberg H., Santos N. Rotavirus serotype G5 associated with diarrhea in Brazilian children. J Clin Microbiol. 1994 May;32(5):1408–1409. doi: 10.1128/jcm.32.5.1408-1409.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Huang J. A., Nagesha H. S., Holmes I. H. Comparative sequence analysis of VP4s from five Australian porcine rotaviruses: implication of an apparent new P type. Virology. 1993 Sep;196(1):319–327. doi: 10.1006/viro.1993.1482. [DOI] [PubMed] [Google Scholar]
  18. Huang J., Nagesha H. S., Dyall-Smith M. L., Holmes I. H. Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses. Arch Virol. 1989;109(3-4):173–183. doi: 10.1007/BF01311079. [DOI] [PubMed] [Google Scholar]
  19. Kang S. Y., Saif L. J., Miller K. L. Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol. 1989 Dec;27(12):2744–2750. doi: 10.1128/jcm.27.12.2744-2750.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ludert J. E., Hidalgo M., Gil F., Liprandi F. Identification in porcine faeces of a novel virus with a bisegmented double stranded RNA genome. Arch Virol. 1991;117(1-2):97–107. doi: 10.1007/BF01310495. [DOI] [PubMed] [Google Scholar]
  21. Mattion N. M., Bellinzoni R. C., Blackhall J. O., La Torre J. L., Scodeller E. A. Antigenic characterization of swine rotaviruses in Argentina. J Clin Microbiol. 1989 Apr;27(4):795–798. doi: 10.1128/jcm.27.4.795-798.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nagesha H. S., Holmes I. H. Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay. J Med Virol. 1991 Nov;35(3):206–211. doi: 10.1002/jmv.1890350311. [DOI] [PubMed] [Google Scholar]
  23. Nagesha H. S., Holmes I. H. New porcine rotavirus serotype antigenically related to human rotavirus serotype 3. J Clin Microbiol. 1988 Feb;26(2):171–174. doi: 10.1128/jcm.26.2.171-174.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nagesha H. S., Holmes I. H. VP4 relationships between porcine and other rotavirus serotypes. Arch Virol. 1991;116(1-4):107–118. doi: 10.1007/BF01319235. [DOI] [PubMed] [Google Scholar]
  25. Nagesha H. S., Huang J., Holmes I. H. A variant serotype G3 rotavirus isolated from an unusually severe outbreak of diarrhoea in piglets. J Med Virol. 1992 Oct;38(2):79–85. doi: 10.1002/jmv.1890380202. [DOI] [PubMed] [Google Scholar]
  26. Nagesha H. S., Huang J., Hum C. P., Holmes I. H. A porcine rotavirus strain with dual VP7 serotype specificity. Virology. 1990 Mar;175(1):319–322. doi: 10.1016/0042-6822(90)90215-d. [DOI] [PubMed] [Google Scholar]
  27. Paul P. S., Lyoo Y. S., Andrews J. J., Hill H. T. Isolation of two new serotypes of porcine rotavirus from pigs with diarrhea. Arch Virol. 1988;100(1-2):139–143. doi: 10.1007/BF01310917. [DOI] [PubMed] [Google Scholar]
  28. Pongsuwanna Y., Taniguchi K., Choothanom M., Chiwakul M., Jayavasu C., Snodgrass D. R., Urasawa S. Serological and genetic characterization of bovine rotaviruses in Thailand by ELISA and RNA-RNA hybridization: detection of numerous non-serotype 6 strains. Southeast Asian J Trop Med Public Health. 1990 Dec;21(4):607–613. [PubMed] [Google Scholar]
  29. Pongsuwanna Y., Taniguchi K., Wakasugi F., Sutivijit Y., Chiwakul M., Warachit P., Jayavasu C., Urasawa S. Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR. Epidemiol Infect. 1993 Oct;111(2):407–412. doi: 10.1017/s0950268800057095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Pongsuwanne Y., Taniguchi K., Choonthanom M., Chiwakul M., Susansook T., Saguanwongse S., Jayavasu C., Urasawa S. Subgroup and serotype distributions of human, bovine, and porcine rotavirus in Thailand. J Clin Microbiol. 1989 Sep;27(9):1956–1960. doi: 10.1128/jcm.27.9.1956-1960.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rosen B. I., Parwani A. V., Lopez S., Flores J., Saif L. J. Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. J Clin Microbiol. 1994 Feb;32(2):311–317. doi: 10.1128/jcm.32.2.311-317.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Saif L. J., Jiang B. Nongroup A rotaviruses of humans and animals. Curr Top Microbiol Immunol. 1994;185:339–371. doi: 10.1007/978-3-642-78256-5_11. [DOI] [PubMed] [Google Scholar]
  33. Shen S., Burke B., Desselberger U. Nucleotide sequences of the VP4 and VP7 genes of a Chinese lamb rotavirus: evidence for a new P type in a G10 type virus. Virology. 1993 Nov;197(1):497–500. doi: 10.1006/viro.1993.1620. [DOI] [PubMed] [Google Scholar]
  34. Svensson L., Padilla-Noriega L., Taniguchi K., Greenberg H. B. Lack of cosegregation of the subgroup II antigens on genes 2 and 6 in porcine rotaviruses. J Virol. 1990 Jan;64(1):411–413. doi: 10.1128/jvi.64.1.411-413.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Taniguchi K., Urasawa T., Morita Y., Greenberg H. B., Urasawa S. Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987 Jun;155(6):1159–1166. doi: 10.1093/infdis/155.6.1159. [DOI] [PubMed] [Google Scholar]
  36. Taniguchi K., Urasawa T., Pongsuwanna Y., Choonthanom M., Jayavasu C., Urasawa S. Molecular and antigenic analyses of serotypes 8 and 10 of bovine rotaviruses in Thailand. J Gen Virol. 1991 Dec;72(Pt 12):2929–2937. doi: 10.1099/0022-1317-72-12-2929. [DOI] [PubMed] [Google Scholar]
  37. Taniguchi K., Urasawa T., Urasawa S. Independent segregation of the VP4 and the VP7 genes in bovine rotaviruses as confirmed by VP4 sequence analysis of G8 and G10 bovine rotavirus strains. J Gen Virol. 1993 Jun;74(Pt 6):1215–1221. doi: 10.1099/0022-1317-74-6-1215. [DOI] [PubMed] [Google Scholar]
  38. Taniguchi K., Urasawa T., Urasawa S. Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains. Virology. 1994 May 1;200(2):390–400. doi: 10.1006/viro.1994.1203. [DOI] [PubMed] [Google Scholar]
  39. Taniguchi K., Wakasugi F., Pongsuwanna Y., Urasawa T., Ukae S., Chiba S., Urasawa S. Identification of human and bovine rotavirus serotypes by polymerase chain reaction. Epidemiol Infect. 1992 Oct;109(2):303–312. doi: 10.1017/s0950268800050263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Urasawa S., Hasegawa A., Urasawa T., Taniguchi K., Wakasugi F., Suzuki H., Inouye S., Pongprot B., Supawadee J., Suprasert S. Antigenic and genetic analyses of human rotaviruses in Chiang Mai, Thailand: evidence for a close relationship between human and animal rotaviruses. J Infect Dis. 1992 Aug;166(2):227–234. doi: 10.1093/infdis/166.2.227. [DOI] [PubMed] [Google Scholar]
  41. Urasawa S., Urasawa T., Taniguchi K., Chiba S. Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Arch Virol. 1984;81(1-2):1–12. doi: 10.1007/BF01309292. [DOI] [PubMed] [Google Scholar]
  42. Urasawa T., Taniguchi K., Kobayashi N., Mise K., Hasegawa A., Yamazi Y., Urasawa S. Nucleotide sequence of VP4 and VP7 genes of a unique human rotavirus strain Mc35 with subgroup I and serotype 10 specificity. Virology. 1993 Aug;195(2):766–771. doi: 10.1006/viro.1993.1428. [DOI] [PubMed] [Google Scholar]

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