Table 3.
Results obtained for diabetic foot ulcer sample using pyrosequencing (PRAPS).
| Genus | Seq | % | Gram | Aerotolerance | Shape |
| Staphylococcus spp. | 10874 | 29.72 | + | Facultative anaerobe | Cocci |
| Peptoniphilus spp. | 2555 | 6.98 | + | Anaerobic | Cocci |
| RhodoPseudomonas spp. | 2541 | 6.94 | - | Facultative anaerobe | Rod |
| Enterococcus spp. | 2341 | 6.40 | + | Facultative anaerobe | Cocci |
| Veillonella spp. | 1978 | 5.41 | - | Anaerobic | Cocci |
| Clostridium spp. | 1975 | 5.40 | + | Anaerobic | Rod |
| Finegoldia magna | 1953 | 5.34 | + | Anaerobic | Cocci |
| Haemophilus spp. | 1701 | 4.65 | - | Facultative anaerobe | Rod |
| Acinetobacter spp. | 1301 | 3.56 | - | Aerobic | Rod |
| Morganella spp. | 1240 | 3.39 | - | Facultative anaerobe | Rod |
| Serratia spp. | 1125 | 3.07 | - | Facultative anaerobe | Rod |
| Proteus spp. | 1072 | 2.93 | - | Facultative anaerobe | Rod |
| Dialister spp. | 1029 | 2.81 | - | Anaerobic | Rod |
| Streptococcus spp. | 751 | 2.05 | + | Facultative anaerobe | Cocci |
| Stenotrophomonas spp. | 669 | 1.83 | + | Aerobe | Rod |
| Peptococcus niger | 588 | 1.61 | + | Anaerobic | Cocci |
| UWB | 342 | 0.93 | unk | Unk | Unk |
| Klebsiella spp. | 326 | 0.89 | - | Facultative anaerobe | Rod |
| Actinomyces spp. | 307 | 0.84 | + | Facultative anaerobe | Rod |
| Gordonia spp. | 302 | 0.83 | + | Aerobic | Rod |
| Delftia spp. | 251 | 0.69 | - | Aerobic | Rod |
| Gemella spp. | 168 | 0.46 | + | Anaerobic | Cocci |
| Corynebacterium spp. | 157 | 0.43 | + | Facultative anaerobe | Rod |
| UWB | 143 | 0.39 | unk | Unk | Unk |
| UWB | 107 | 0.29 | unk | Unk | Unk |
| Salmonella enterica | 102 | 0.28 | - | Facultative anaerobe | Rod |
| Fusobacterium spp. | 99 | 0.27 | - | Anaerobic | Rod |
| Varibaculum cambriense | 54 | 0.15 | + | Anaerobic | Rod |
| Enterobacter spp. | 52 | 0.14 | - | Facultative anaerobe | Rod |
| Bacillus spp. | 51 | 0.14 | + | aerobic | Rod |
| Eikenella spp. | 42 | 0.11 | - | facultative anaerobe | Rod |
| Anaerococcus spp. | 42 | 0.11 | + | anaerobic | Cocci |
| Hydrogenophaga spp. | 40 | 0.11 | - | aerobic | Rod |
| Alcaligenes faecalis | 36 | 0.10 | - | aerobic | Rod |
| E coli | 32 | 0.09 | - | facultative anaerobe | Rod |
| Sphingomonas spp. | 26 | 0.07 | - | aerobic | Rod |
| Acidovorax spp. | 26 | 0.07 | - | aerobic | Rod |
| Prevotella spp. | 22 | 0.06 | - | anaerobic | Rod |
| UWB | 20 | 0.05 | unk | unk | Unk |
| Eubacterium spp. | 20 | 0.05 | + | anaerobic | Rod |
| Bacteroides spp. | 20 | 0.05 | - | anaerobic | Rod |
| UWB | 17 | 0.05 | unk | unk | Unk |
| UWB | 16 | 0.04 | unk | unk | Unk |
| Selenomonadaceae spp. | 16 | 0.04 | - | anaerobic | Rod |
| Brevibacterium spp. | 14 | 0.04 | + | aerobic | Rod |
| Riemerella spp. | 13 | 0.04 | - | aerobic | Rod |
| UWB | 11 | 0.03 | unk | unk | Unk |
| Bradyrhizobium spp. | 11 | 0.03 | - | aerobic | Rod |
| Pantoea agglomerans | 10 | 0.03 | - | facultative anaerobe | Rod |
This table provides the identified genus of the bacteria found using pyrosequencing (PRAPS) in the D sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. * The asterisk identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the diabetic foot ulcer pool (D sample). Bacteria that were cultured and were not found using PRAPS include Citrobacter and Pseudomonas. % represents the percentage of the total sequences analyzed within the sample.