Table 4.
Results obtained for the pressure ulcer sample (P) using pyrosequencing (PRAPS).
Genus | Seq | % | Gram | Aerotolerance | Shape |
Peptoniphilus spp. | 10543 | 38.38 | + | Anaerobe | Cocci |
Serratia spp. | 5234 | 19.05 | - | facultative anaerobe | Rod |
Peptococcus niger. | 3042 | 11.07 | + | anaerobe | Cocci |
Streptococcus spp. | 3016 | 10.98 | + | facultative anaerobe | Cocci |
Finegoldia magna | 1743 | 6.34 | + | anaerobe | Cocci |
Dialister spp. | 1374 | 5.00 | - | anaerobe | rod |
Pectobacterium spp. | 528 | 1.92 | - | facultative anaerobe | rod |
Enterobacter spp. | 392 | 1.43 | - | facultative anaerobe | rod |
Proteus spp. | 308 | 1.12 | - | facultative anaerobe | rod |
Veillonella spp. | 186 | 0.68 | - | anaerobe | cocci |
UWB | 141 | 0.51 | Unk | unk | unk |
UWB | 121 | 0.44 | Unk | unk | unk |
Clostridium spp. | 93 | 0.34 | + | anaerobe | rod |
Corynebacterium striatum | 73 | 0.27 | + | aerobe | rod |
Delftia spp. | 65 | 0.24 | - | aerobe | rod |
UWB | 63 | 0.23 | Unk | unk | unk |
Enterococcus spp. | 62 | 0.23 | + | facultative anaerobe | cocci |
Staphylococcus spp. | 56 | 0.20 | + | facultative anaerobe | cocci |
Hydrogenophaga spp. | 54 | 0.20 | - | aerobe | rod |
Eggerthella lenta | 33 | 0.12 | + | anaerobe | rod |
UWB | 31 | 0.11 | Unk | unk | unk |
UWB | 31 | 0.11 | Unk | unk | unk |
Prevotella spp. | 31 | 0.11 | - | anaerobe | rod |
Varibaculum cambriense | 28 | 0.10 | + | anaerobe | rod |
Actinomyces europaeus | 28 | 0.10 | + | facultative anaerobe | rod |
Ferrimonas spp. | 27 | 0.10 | - | facultative anaerobe | rod |
Bacillus spp. | 24 | 0.09 | + | aerobe | rod |
UWB | 22 | 0.08 | Unk | unk | unk |
Fusobacterium spp. | 22 | 0.08 | - | anaerobe | rod |
Alcaligenes faecalis | 20 | 0.07 | - | aerobe | rod |
UWB | 17 | 0.06 | Unk | unk | unk |
Riemerella spp. | 15 | 0.05 | - | aerobe | rod |
UWB | 14 | 0.05 | Unk | unk | unk |
Stenotrophomonas spp. | 14 | 0.05 | - | aerobe | rod |
Shewanella spp. | 11 | 0.04 | - | facultative anaerobe | rod |
Eubacterium spp. | 10 | 0.04 | + | anaerobe | rod |
This table provides the identified genus of the bacteria found using pyrosequencing (PRAPS) in the P sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. * The asterisk identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using PRAPS include Acinetobacter, Leclercia, Morganella, and Pseudomonas. The % represents the percentage of the total sequences analyzed within the sample.