Table 8.
Results obtained for the pressure ulcer sample (P) using shotgun Sanger sequencing (FRACS).
Genus species | Seq | % | Gram | Aerotolerance | Shape |
Peptoniphilus ivorii | 51 | 27.42 | + | anaerobic | cocci |
Anaerococcus lactolyticus | 20 | 10.75 | + | anaerobic | cocci |
Anaerococcus vaginalis | 16 | 8.60 | + | anaerobic | cocci |
Streptococcus dysgalactiae* | 14 | 7.53 | + | facultative | cocci |
Peptoniphilus harei | 14 | 7.53 | + | anaerobic | cocci |
Peptococcus niger | 12 | 6.45 | + | anaerobic | rod |
Serratia marcescens | 12 | 6.45 | - | aerobic | rod |
Finegoldia magna | 10 | 5.38 | + | anaerobic | cocci |
Peptoniphilus indolicus | 7 | 3.76 | + | anaerobic | cocci |
Clostridium hathewayi | 4 | 2.15 | + | anaerobic | rod |
Prevotella buccalis | 3 | 1.61 | - | anaerobic | rod |
Pseudomonas aeruginosa* | 3 | 1.61 | - | aerobic | rod |
UWB | 2 | 1.08 | Unk | unk | unk |
Dialister invisus | 2 | 1.08 | - | anaerobic | rod |
Dialister spp. | 2 | 1.08 | - | anaerobic | rod |
Delftia acidovorans | 2 | 1.08 | - | aerobic | rod |
Pseudomonas fluorescens* | 2 | 1.08 | - | aerobic | rod |
Helcococcus kunzii | 1 | 0.54 | + | facultative anaerobe | cocci |
Enterococcus faecalis* | 1 | 0.54 | + | facultative | cocci |
Staphylococcus epidermidis* | 1 | 0.54 | + | facultative | cocci |
Streptococcus pyogenes* | 1 | 0.54 | + | facultative | cocci |
Clostridium perfringens | 1 | 0.54 | + | anaerobic | rod |
Klebsiella granulomatis | 1 | 0.54 | - | Facultative anaerobe | rod |
Klebsiella pneumoniae | 1 | 0.54 | - | Facultative anaerobe | rod |
Proteus vulgaris* | 1 | 0.54 | - | Facultative anaerobe | rod |
Porphyromonas uenonis | 1 | 0.54 | - | anaerobic | rod |
Pseudomonas spp.* | 1 | 0.54 | - | aerobic | rod |
This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the P sample, the number of sequences corresponding to the given genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using FRACSS include Acinetobacter, Enterobacter, Leclercia and Morganella. The % represents the percentage of the total sequences analyzed within the sample.