Table 1.
| Protein Name | Full Name | Ortholog | MW (kDa) | Mitotic Localization | Activity |
|---|---|---|---|---|---|
| Astrin hMAP126 Spag5 DEEPEST | Aster associated protein | M.musculus (Spag5) | ∼135 | spindle poles, kinetochores of bioriented chromosomes | Crosslinks and stabilizes microtubules; Stabilizes cohesin (ref 17, 18) |
| HURP DLG7 | Hepatoma Up-Regulated Protein | C.elegans, D.melanogaster, M.musculus, S.cerevisiae, X.laevis | ∼95 | Kinetochore fibers, adjacent to kinetochores | Stabilizes kinetochore fibers; contributes to chromosome alignment (ref 10) |
| NuMA | NUclear Mitotic Apparatus protein | D.melanogaster (Mud, Asp1*), M.musculus, X.laevis | ∼215 | Spindle Poles | Required for the formation and maintenance of focused spindle poles; part of a complex that inhibits APC/C at spindle poles (ref 4, 40) |
| NuSAP | NUcleolar Spindle-Associated Protein | M.musculus, X.laevis | ∼55 | Central Spindle | Contributes to Nucleation, stabilization and bundling of microtubules near chromosomes; (ref 14, 15, 16) |
| PRC1 | Protein Regulator of Cytokinesis | C.elegans (SPD-1), D.melanogaster (Fascetto/Feo), M.musculus, S.cerevisae (Ase1), S.pombe (Ase1), X.laevis | ∼70 | Central spindle, spindle midbody | Cross-links antiparellel spindle midzone microtubules to stabilize the elongating spindle during anaphase (ref 11, 12) |
| RHAMM HMMR | Receptor of HyAluronon-Mediated Motility | M.musculus, X.laevis (XRHAMM) | ∼70 | Centrosomes, spindle poles, spindle midzone | Induces microtubule nucleation and stability at spindle poles; influences Cyclin B1 activity (ref 26, 28, 39) |
| TACC (1, 2, 3) | Transforming Acidic Coiled-Coil protein | C.elegans (TAC-1), D.melanogaster (D-TACC), M.musculus, X.laevis (Maskin) | ∼150 | Centrosomes, Spindle poles | Promotes microtubule nucleation and stabilization at spindle poles (ref 22, 23, 24, 25, 26) |
| TOGp | Tumor Over-expressed Gene | C.elegans (ZYG-9), D.melanogaster (Minispindles/Msps), M.musculus, S.cerevisae (Stu2), S.pombe (Dis1/Alp14), X.laevis (XMAP215/Dis1) | ∼215 | Centrosomes, Spindle poles | Promotes plus end microtubule dynamics; promotes centrosome and spindle pole stability (ref 19, 20, 21) |
| TPX2 | Targeting Protein for Xklp2 | C.elegans (TPXL-1), D.melanogaster (Asp1*), M.musculus, X.laevis | ∼95 | Spindle Poles, midbody | Cross-links microtubules at poles; promotes microtubule nucleation and maintenance of centrosome integrity; activates AurA (ref 5, 6, 35) |
*
Asp1 has been shown to localize to spindle poles where it has microtubule nucleation activities similar to that of TPX2 and crosslinking activity involved in spindle pole focusing similar to that of NuMA