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. 2002 Jan 1;538(Pt 1):53–63. doi: 10.1113/jphysiol.2001.012794

Figure 6. Single-cell RT-PCR performed on axotomised motoneurons in rat spinal cord slices.

Figure 6

A, ethidium bromide-stained agarose gel electrophoresis of PCR product amplified with primers specific for NR2A. The product was digested with the restriction enzyme Bmp I. B, PCR product amplified with primers specific for NR2B and digested with Bfa I. C, PCR product amplified with primers specific for NR2C and digested with Sca I. D, PCR product amplified with primers specific for NR2D and NR3A. M: marker DNA (bp).