Figure 3. Stress preconditioning restores the ability of alveolar epithelium to respond to catecholamines by upregulating alveolar fluid clearance after haemorrhage.

Rats were anaesthetized and then either subjected to whole body hyperthermia (42 °C for 20 min 12–16 h before onset of haemorrhage) or injected with geldanamycin (i.p., 1 mg kg⁻¹, 48 and 24 h before onset of haemorrhage). Half of the animals were left untreated while the other half were subjected to haemorrhagic shock as described in Methods. Measurement of alveolar liquid clearance was performed as described in Methods. A, alveolar fluid clearance (% of instilled liquid) (mean ± s.e.m.) is shown for control and haemorrhaged rats that were or were not pretreated with whole body hyperthermia; B, alveolar fluid clearance (% of instilled liquid) (mean ± s.e.m.) is shown for control and haemorrhaged rats that were or were not pretreated with geldanamycin; *P < 0.05 from haemorrhaged and resuscitated rats that did not receive any adrenaline in their airspaces.