Figure 4. Stress preconditioning via whole body hyperthermia leads to increased expression of Hsp72 protein in the lung.

Rats were left untreated or preconditioned via whole body hyperthermia. Some of the animals were then subjected to haemorrhagic shock. Animals were killed, the lungs removed and then examined for their expression of Hsp72 by Western blotting. A, Western blot analysis for Hsp72. One representative lung is shown for each experimental condition. Three additional experiments gave comparable results. B, densitometry analysis of four comparable Western blots for detection of Hsp72 protein showed a significant increase in the expression of Hsp72 protein in lung homogenate from rats that underwent whole body hyperthermia compared with non-pretreated rats; *P < 0.05 from control rats that did not receive any pretreatment; ** P < 0.05 from haemorrhaged and resuscitated rats that did not receive any pretreatment.