Figure 5. Stress preconditioning using geldanamycin leads to increased expression of Hsp72 in the lung.

Rats were left untreated or injected with geldanamycin. Then the animals were killed and the lungs, liver and kidneys were removed and examined for their expression of Hsp72 by Western blotting. Equal amounts of total protein were applied to the gel. A, Western blot analysis for Hsp72 protein in control rats that have or have not been pretreated with geldanamycin. One representative experiment is shown for each experimental condition. Three additional experiments gave comparable results. B, densitometry analysis of four comparable Western blots for detection of Hsp72 protein showed a significant increase in the expression of Hsp72 protein in lung homogenate from rats that were pretreated with geldanamycin compared with non-pretreated rats; *P < 0.05 from control rats that did not receive any pretreatment; **P < 0.05 from the values measured in the lung.