Figure 9. Increased expression of Hsp72 protein production by A549 or primary cultures of rat ATII cell monolayers after stress preconditioning with heat.

Control cells were maintained at 37 °C. A group of cells were stress preconditioned with heat (43 °C for 60 min for A549 cells and 30 min for ATII cells) 12 h before being harvested. A, Western blot analysis for Hsp72 protein expression in A549 cell monolayers that were or were not stress preconditioned with heat. One representative experiment is shown for each experimental condition. Three additional experiments gave comparable results. B, Western blot analysis for Hsp72 protein expression in primary cultures of rat ATII cell monolayers that were or were not stress preconditioned with heat. One representative experiment is shown for each experimental condition. Three additional experiments gave comparable results; *P < 0.05 from cell monolayers that were not stress preconditioned with heat.