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. 2002 Jan 15;538(Pt 2):483–493. doi: 10.1113/jphysiol.2001.013175

Figure 1. Mechanical stimulation and experimental protocols.

Figure 1

A, a representation of one experimental trial consisting of an ascending series of four 2 s ‘ramp and hold’ mechanical stimuli (S1–4) applied to the most sensitive spot of the receptive field with inter-stimulus intervals of 10 s. The 10 s of recording prior to S1 was used to measure spontaneous activity. The number of action potentials produced by each stimulus is given in parentheses. B, a representative experiment showing the protocol for testing the effect of dbcAMP on mechanical responsiveness. Baseline responses in the presence of SIF were followed by 5 trials in which dbcAMP (cAMP) was applied and another 5 trials of wash with SIF. Note the short duration of dbcAMP action. C, a representative experiment demonstrating the protocol for testing the effect of PKA blockade. The PKA inhibitors Rp-cAMPS or H-89 were applied first, followed by a mixture of dbcAMP and the inhibitor. dbcAMP was applied next for 10 min and followed by a wash period.