A. the effect of the process of differentiation and separation on a 50 ms window of EMG data from the 1DI muscle of a healthy subject. Moving from left to right the EMG is rendered polyphasic by third-order differentiation, then the common component due to electrical cross-talk is removed by separation, finally the polyphasic, separated EMG is reintegrated. Note the similarity between the original EMG signal and the final processed EMG signal. B, the effect of the separation algorithm on the five EMGs recorded from a single subject. The data shown is the average of the unrectified signals, pooled across trials. Note that the EMGs with the largest amount of electrical cross-talk, shown in the difference plot, are the intrinsic hand muscles.